الوصف
Bind a variety of biotinylated ligands, including DNA/RNA, oligos, antibodies and proteins with Thermo Scientific™ Power-Bind Streptavidin Coated Particles. Particles have very high and stable biotin binding capacities and address the concerns of diagnostic test kit manufacturers and nucleic acid researchers. The microparticles reduce serum matrix effects and improve assay accuracy, resulting in low, non-specific binding of serum proteins and other interfering substances.
The use of microparticles as a solid phase support in various immunoassays and affinity purifications has been known for some time; however, achieving high activity and stable binding of solid phase ligands has been a major difficulty. Biotinylated compounds become stably bound to Power-Bind Streptavidin Coated particles after simple incubation in buffer due to the high affinity of the biotin-streptavidin interaction. The particles also function as a spacer, which improves the specific activity of the bound ligand. Monodisperse nonmagnetic particle suspensions with streptavidin covalently bound to the surface in a highly active form
المميزات:
- Made from 0.3μm or 0.8μm Thermo Scientific™ OptiLink™ Carboxylate-Modified particles
- High biotin-binding capacity
- مستقر للغاية، وعمر افتراضي طويل
- Simplifies the process of binding ligands to particles
- Combines the advantages of high surface area and easy, high affinity, high specific activity binding
- Excellent for chemiluminescent assays, biotinylated ligand capture, and for molecular biology, immunoassay, or sample prep applications
الفوائد :
- Dissociation constant (Kd 10-15 molar), easy one-step binding protocols for biotinylated ligands
- High activity of surface bound ligands to easily solve difficult coupling problems
- Simple aqueous biotinylation reactions
- Low nonspecific interactions (blocked microparticle surface)
- Highly mobile particles for membrane-based applications
- Can be used in EIA formats with biotin/enzyme detection systems
- Beneficial spacer effect of SA molecule
- A choice of 0.3μm or 0.8μm diameters for different application requirements
تنصح ل:
- Universal nonmagnetic bead for coupling unique ligands.
- Clinical diagnostics and molecular biology applications.
| المواصفات الخاصه | التفاصيل |
|---|---|
| اسم المنتج | Power-Bind Streptavidin Coated Particles |
| لون | أبيض أو عديم اللون |
| للاستخدام مع (التطبيق) | Biotinylated Ligand Binding, Diagnostic Assays |
| الخامة | البوليسترين |
| نوع المنتج | Streptavidin-Coated Particles |
| ويشمل | Package Insert and Certificate of Analysis |
| حافظ | 0.05% Na Az |
| الكمية | 100 مل |
| Sample Evaluation Packs | Packaged in 1mL bottles at 1% solids (10 mg/mL); individual lots available for evaluation |
| الجرف الحياة | شهرين |
| تغليف خاص | Available in different concentrations and package sizes upon request |
| استقرار | Stable in Guanidinium thiocyanate; DMF and DMSO to 100%; withstands sonication and PCR temperature cycling environments |
| القطر (متري) | شنومك μm |
| وحدة الحجم | كل |
